Osmotic adaptation of sea-urchin spermatozoa.

A monoclonal antibody against the dynein IC1 peptide of sea urchin spermatozoa inhibits the motility of sea urchin, dinoflagellate, and human flagellar axonemes.

To investigate the role of axonemal components in the mechanics and regulation of flagellar movement, we have generated a series of monoclonal antibodies (mAb) against sea urchin (Lytechinus pictus) sperm axonemal proteins, selected for their ability to inhibit the motility of demembranated sperm models. One of these antibodies, mAb D1, recognizes an antigen of 142 kDa on blots of sea urchin ax...

The Physiology of Sea-urchin Spermatozoa

Since Gray showed in 1928 that dilute suspensions of sea-urchin spermatozoa respire at a higher rate, per spermatozoon, than dense suspensions, evidence has been accumulating which suggests that sea-urchin spermatozoa are in different physiological conditions in suspensions of different densities. This paper is concerned with these differences and, in particular, with the effects of rigorous pH...

The Physiology of Sea - Urchin Spermatozoa Catalase

The Physiology of Sea-urchin Spermatozoa Action of Versene

A description of the physiology of sea-urchin spermatozoa requires, among other things, an explanation of two special characteristics which these spermatozoa possess. First, immediately after dilution, the Og uptake per spermatozoon is higher in a dilute than in a dense suspension. Secondly, the decline in sperm motility and fertilizing capacity is more rapid in a dilute than in a dense suspens...

Sperm Receptivity in Sea Urchin Oocytes and Eggs

An electrophysiological method for studying the receptivity to spermatozoa of sea urchin eggs and oocytes is described. Pairs of adjacent oocytes and eggs, with intact jelly layers, were impaled and simultaneously exposed to a known concentration of spermatozoa. Two parameters were studied. The time from insemination to the first successful collision (as indicated by a step depolarization acros...